S5a protein
WebJul 7, 2013 · S5a/Rpn10 drives dendrite growth and elaboration in primary neurons and in the rodent brain in vivo We characterized the function of the key proteasomal lid-associated protein S5a/Rpn10 in the mammalian brain, utilizing granule neurons of the developing rat cerebellar cortex. WebFeb 19, 2005 · Proteasome subunit S5a contains two ubiquitin-interacting motifs (UIMs) through which it recruits ubiquitylated substrates to the proteasome for their degradation. …
S5a protein
Did you know?
WebDownload scientific diagram DNA and protein sequence of S5a. The sequence depicted encodes the entire S5a protein beginning with potential initiation Met codons at positions … WebS5a/PSMD4 Antibody detects endogenous levels of total S5a/PSMD4 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly344 of human S5a/PSMD4 protein.
WebDec 15, 2009 · Decreased 19S-S5a protein levels and MGO-modified 20S-β2 proteasome subunit in GLO1-knockdown (GL01-KD) and STZ diabetic mice. Kidney lysate protein (40 … WebThus, the S5a protein provides a general model for studying recognition of polyubiquitin chains by a 26 S protease component. S5a retains the ability to bind ubiquitin chains even after it has been separated on a SDS-acrylamide gel and transferred to nitrocellulose.
WebSep 24, 1999 · S5a is known as a multiubiquitin chain-binding protein. Since both hHR23 proteins have highly conserved Ub-related sequences on NH 2 termini ( 1, 2 ), we speculated that these sequences are involved in the interaction with S5a. Table I. Protein-protein interactions assessed by the yeast two-hybrid system WebOct 14, 2013 · S5a is required for the degradation of p53 but not its E3 ligase Mdm2. Additional work is required to completely define the pathways of proteasomal recognition …
WebThe human protein shares 96% and 99% aa sequence identity with its mouse and rat orthologs, respectively. Structurally, it contains an N-terminal von Willebrand Factor type A domain and two C-terminal Ubiquitin-interacting motifs (UIM). It acts as a Ubiquitin-binding protein where it is most commonly referred to as S5a or in yeast as Rpn10.
WebOct 13, 2003 · The Binding Surfaces of S5a and the UBA Domains Overlap. We used an S5a construct spanning residues M196 to D307 to study the effect of S5a-binding on hHR23a protein structure. S5a (196–307) contains the two ubiquitin-binding domains and was previously shown to bind hHR23a with equal affinity to full-length S5a . the 1 i love lyricsWebApr 10, 2024 · Author summary Critical cellular processes require spatiotemporal regulation of sphingolipid levels among organelle membranes. Programmed cell death and inflammation, for example, are impacted by the distribution of ceramide-1-phosphate (C1P). C1P levels are specifically altered by ceramide-1-phosphate transfer protein (CPTP), … the1group holding gmbhWebS5A antibody; S5a/antisecretory factor protein antibody; see all. Images. Western blot - Anti-Proteasome 19S S5A/ASF antibody (ab18512) Crude Drosophila extract. Secondary antibody: rabbit polyclonal 1:5000. Please note: Drosophila S5a has an actual mass of 42.6 kDa, however, on the gel it runs at an apparent 54 kDa band. the 1 hokage narutoWebJun 15, 2015 · The HIS-tagged human S5a protein was expressed in bacteria following cloning of the entire open reading frame (377 aa) in the pET-21a (Novagen, Billerica, MA) vector. The plasmids were transformed into E. coli BL21 (DE3) and protein expression was induced with 1.0 mM IPTG for 4 hrs at 37°C. the 1 in 1 2 3 : abbrWebApr 23, 2009 · S5a, fusion proteins containing S5a's UIMs or HHR23A was added to ubiquitination mixture from the outset and GST was added as a control of GST-UIM. For the reaction with GST and GST-UIM, a different batch of enzymes (E1, E2 and CHIP) from other reactions was used. the 1 hotel los angelesWebOct 13, 2003 · In this work, we illustrate how the proteasomal subunit S5a regulates hHR23a protein structure. By using NMR spectroscopy, we have elucidated the structure and … the 1 improvementWebIdentification of the Ub-like sequence as the S5a-interacting domain of hHR23B raised the possibility that the S5a protein binds to any proteins which are conjugated with ubiquitin. To examine whether this is the case, we performed competition experiments using either free ubiquitin itself or the NH 2-terminal Ub-like domain of hHR23B. the 1 in 3/1