Flag-tag mouse monoclonal antibody
WebTHE™ DYKDDDDK Tag Antibody, mAb, Mouse THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes DYKDDDDK tags localized at the C-terminal, N-terminal and internal region of DYKDDDDK tagged fusion proteins. Product Details Citations Documents Overview Applications Properties Examples Background For laboratory research use only. WebKey features and details PE Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt, Sandwich ELISA Reacts with: Species independent Conjugation: PE. Ex: 488nm, Em: 575nm Isotype: IgG1 Get better batch-to-batch reproducibility with a recombinant antibody
Flag-tag mouse monoclonal antibody
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Web> 无血清快速细胞冻存液. QuickFreezing-M. QuickFreezing-2*M WebDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) DDDDK tag Monoclonal Antibody for ELISA. Host / Isotype. Mouse / IgG2b. Reactivity. human, mouse, rat . ... Monoclonal ANTI-FLAG? M2 …
WebJan 21, 2024 · For the primary antibody reaction, DYKDDDDK Tag (D6W5B) Rabbit monoclonal antibody (1:1000, 14793; Cell Signal Technology, Beverly, MA, USA) and anti-C23 (NCL) mouse monoclonal (1:500, sc-55486; Santa Cruz Biotechnology, Dallas, TX, USA) were used in the antibody solution, and the mixture was allowed to react with the … WebThe Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides. Packaging 0.2, 1, 1 mg Application
WebOct 4, 2024 · The FLAG tags are attached to the N-terminus or C-terminus of the target proteins. The tagged protein binds to the specific monoclonal antibodies conjugated on the matrix of magnetic beads or agarose gel. The residual impurities are washed off using the reagent washing buffer. WebThis antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology. Protocols Standard Protocols Click here to view our Standard Protocols Publications All (610) WB …
WebJun 15, 2012 · The FLAG sequence (DYKDDDDK) is an artificial sequence widely used to detect, quantify, and purify proteins expressed as FLAG-fusion proteins. Several highly specific monoclonal antibodies for FLAG are commercially available; however, they are not always sensitive enough to detect proteins expressed …
WebFlag Tag monoclonal antibody CSB-MA000021M0m was produced in the mouse immunized by using the DYKDDDDK(Flag) synthetic peptide conjugate to KLH as the … fisheries observer salaryWebKey features and details. PerCP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt. Reacts with: Species independent. Conjugation: PerCP. Ex: 482nm, Em: 675nm. Isotype: IgG1. fisheries observer trainingWebMonoclonal ANTI-FLAG ® M1 antibody produced in mouse is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells. … fisheries observer programhttp://www.abpbiosciences.com/product/flag-tag/ canadian infantry officer mosidWebDYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic … canadian infantry platoon weaponsWebPolyclonal Antibody for studying Flag-Tag. Cited in 708 publications. Validated for WB, IP, F. Available in 2 sizes. ... (G3A1) mAb IgG1 Isotype Control #5415 for mouse monoclonal IgG1 primary antibodies, Mouse … canadian infection controlWeb3 hours ago · Plates were washed as before and incubated with an HRP-conjugated anti-FLAG antibody (clone M2, Sigma-Aldrich, Burlington, MA, USA, 1:10.000 dilution) for 1 h at RT. ... or an established mouse IgG2 monoclonal antibody recognizing PSD95. In these indirect setups, after washing (3 × 5 min in PBS each), the anti-PSD95 r-hcAb or … canadian infantry soldier salary